Publication. SBC

Title:

Altering channel forming activity of syringomycin E via membrane dipole potential

Author:

Ostroumova O.S., Malev V.V., Bessonov A.N., Takemoto J.Y., Schagina L.V

Journal:

Langmuir

Abstract:

The membrane dipole potential is responsible for the modulation of numerous biological processes. It was previously shown (Ostroumova, O. S.; Kaulin, Y. A.; Gurnev, P. A.; Schagina, L. V. Langmuir 2007, 23, 6889-6892) that variations in the dipole potential lead to changes in the channel properties of the antifungal lipodepsipeptide syringomycin E (SRE). Here, data are presented demonstrating the effect of the membrane dipole potential on the channel-forming activity of SRE. A rise in the dipole potential is accompanied by both an increase in the minimum SRE concentration required for the detection of single channels at fixed voltage and a decrease in the steady-state number of open SRE channels at a given SRE concentration and voltage. These alterations are determined by several factors: gating charge, connected with translocations of lipid and SRE dipoles during channel formation, the bilayer-water solution partitioning of SRE, and the chemical work related to conformational changes during channel formation.

Citation:

Ostroumova O.S., Malev V.V., Bessonov A.N., Takemoto J.Y., Schagina L.V. 2008. Altering channel forming activity of syringomycin E via membrane dipole potential. Langmuir 24: 2987-2991

Hyperlink:

http://www.ncbi.nlm.nih.gov/pubmed/18324870?dopt=Abstract